Isolation, characterisation and mapping of simple
sequence repeat loci in potato
Dan Milbourne1, Rhonda C. Meyer1, Angela J.
Collins1,4, Luke D. Ramsay1,Christiane Gebhardt3 and
Robbie Waugh2
1
Department of Cell and Molecular Genetics and 2
Department of Crop Genetics, Scottish Crop Research Institute, Invergowrie, Dundee DD2
5DA, Scotland, UK
3 Max-Planck-Institut für Züchtungforschung, Carl-von-Linné-Weg 10, D-50829,
Köln, Germany
4Current Address: Department of Plant Sciences, University of Oxford, South
Parks Road, Oxford, OX1 3RB, UK.
Corresponding author: Dr Robbie Waugh (address as above)
Tel: 01382 562731; Fax: 01382 562426
E-mail: rwaugh@scri.sari.ac.uk
If you make use of these SSRs, we would appreciate feedback on map
locations, alleles sizes, PIC values etc. so that we can coordinate and update the
informationavailable through these pages. Please send such information to either Robbie Waugh or David Marshall
Fig 1 Potato Linkage Map showing location of mapped SSRs
Beware this file is large
!!!!
Table 1. Oligonucleotide sequences and
associated information for the 112 primer pairs.
CODE
|
EMBL#
|
REPEAT
|
|
PRIMER SEQUENCE 5' - 3'
|
Tm
(oC)
|
PROD
(bp)
|
QUALITY
SCORE
|
LOCATION
|
LG
|
| STM0001 |
|
(TG)4 (TC)2 (TG)5 |
F |
AGTATTCAACCCATTGACTTGGA |
59.8 |
170 |
2 |
|
VI |
| |
|
|
R |
TAGACAAGCCAAGCTGGAGAA |
60.1 |
80--175 |
|
|
|
| STM0002 |
|
(TA)8 (TG)8 TC (TG)7 |
F |
GTTTCAATCTCCAGCCAA |
54.5 |
130 |
3 |
|
|
| |
|
|
R |
AGATGTACTATCTAGCCCACG |
53.7 |
|
|
|
|
| STM0003 |
|
(AC)9 (AT)9 |
F |
GGAGAATCATAACAACCAG |
50.2 |
141 |
2 |
|
XII |
| |
|
|
R |
AATTGTAACTCTGTGTGTGTG |
50.9 |
|
|
|
|
| STM0004 |
|
(AC)9 (AT)7 (AC)5 |
F |
CGAGGGCGTAAACTCATGATA |
60.1 |
149 |
2 |
|
VII |
| |
|
|
R |
AGGTTATTGTGGACACAGTCTTCA |
60.0 |
|
|
|
|
| STM0006 |
|
(AC)14 (AT)5 |
F |
GAAGTTGACATTGAGCCC |
53.8 |
112 |
3 |
|
|
| |
|
|
R |
GGATATCCATTCTTAGATGCA |
54.4 |
|
|
|
|
| STM0007 |
|
(AC)9 |
F |
GGACAAGCTGTGAAGTTTAT |
52.0 |
178 |
1 |
|
XII |
| |
|
|
R |
AATTGAGAAAGAGTGTGTGTG |
52.7 |
|
|
|
|
| STM0010 |
|
(TG)7 (TA)6 (TG)4 TA(TG)5 |
F |
TCCTTATATGGAGCAAGCA |
54.4 |
174 |
2 |
|
IX |
| |
|
|
R |
CCAGTAGATAAGTCATCCCA |
52.6 |
|
|
|
|
| STM0011 |
|
(TG)7 (TA)6 |
F |
CACCTTTTTCTGTTGATGG |
54.0 |
174 |
2 |
|
|
| |
|
|
R |
ATAATATGTGTGACTCGCCA |
54.0 |
|
|
|
|
| STM0013 |
|
(AC)23 (AT)6 (AG)9 |
F |
AACTATCAACTAAATGCCTTTTT |
53.6 |
195 |
2 |
|
V |
| |
|
|
R |
TTAATATTTTTTACTCGGCTATTG |
54.3 |
130 |
|
|
|
| STM0014 |
|
(GT)5 (AT)7 (GT)10 |
F |
CAGTCTTCAGCCCATAGG |
54.1 |
180 |
3 |
|
VII,XII |
| |
|
|
R |
TAAACAATGGTAGACAAGACAAA |
54.3 |
|
|
|
|
| STM0015 |
|
(AC)13 (AT)10 |
F |
GATTGTGAGAAGGCACTGA |
54.5 |
193 |
1 |
|
|
| |
|
|
R |
CACTTGATATACTAGTGTGTTTGG |
53.5 |
|
|
|
|
| STM0017 |
|
(AC)3 (AT)2 (AC)4 |
F |
ATCATGATGACACCTACTATAACC |
54.0 |
176 |
2 |
|
IX |
| |
|
(AT)6 (AC)7 |
R |
TCCACACCTCTATCTGTTGA |
54.0 |
|
|
|
|
| STM0019 |
|
(AT)7 (GT)10 (AT)4 (GT)5 (GC)4 (GT)4 |
F |
AATAGGTGTACTGACTCTCAATG |
52.8 |
204 |
2 |
|
VI |
| |
|
|
R |
TTGAAGTAAAAGTCCTAGTATGTG |
52.6 |
|
|
|
|
| STM0020 |
|
(AC)10 |
F |
AGTCCAGAAAACCACATACA |
53.0 |
176 |
2 |
|
|
| |
|
|
R |
TGCGTCTGTGTGAGTATATTT |
53.4 |
|
|
|
|
| STM0021 |
|
(GT)11 (G)14 |
F |
TCTAGATAAATCACACACTTCCA |
54.2 |
121 |
2 |
|
|
| |
|
|
R |
AATGTCCCGAGTTGGATA |
53.6 |
|
|
|
|
| STM0023 |
|
(TG)3 (TA)6 (TG)12 |
F |
TTTTTGTATGTTTCAACCTCTAG |
53.3 |
132 |
1 |
|
|
| |
|
|
R |
GAAAGATGTACTATCTAGCCGA |
53.2 |
|
|
|
|
| STM0024 |
|
(GTT)5 ... (AC)12 (AT)6 |
F |
CATTACCTTGTGAGATTAGATTG |
53.4 |
135 |
2 |
|
VIII |
| |
|
|
R |
CATATAAGTAGGAATAGGAGGTTT |
52.3 |
|
|
|
|
| STM0025 |
|
(TG)13 |
F |
GTTCATGATTGTGAATGCTC |
53.9 |
146 |
2 |
|
XI |
| |
|
|
R |
ATGACTCAACCCCAAATG |
54.0 |
|
|
|
|
| STM0028 |
|
(AC)12 (AT)5 (AG)8 |
F |
CATAAATGGTTATACACGCTTTGC |
59.8 |
145 |
2 |
|
VII |
| |
|
|
R |
TAATGGAGTTCCTGAAAAGAAAGG |
60.0 |
|
|
|
|
| STM0030 |
|
compound (GT/GC) GT)8 |
F |
AGAGATCGATGTAAAACACGT |
54.0 |
147 |
2 |
|
XII |
| |
|
|
R |
GTGGCATTTTGATGGATT |
54.1 |
|
|
|
|
| STM0031 |
|
(AC)5 ... (AC)3 (GCAC)..(AC)2 (GCAC)2 |
F |
CATACGCACGCACGTACAC |
59.8 |
172 |
2 |
|
VII |
| |
|
|
R |
TTCAACCTATCATTTTGTGAGTCG |
60.4 |
|
|
|
|
| STM0032 |
|
(AC)7 ... (AC)5 |
F |
GGCTGCAGGAATTATGTGTTC |
59.6 |
174 |
1 |
|
XII |
| |
|
|
R |
GATGTAAAACACGTGTGCGTG |
60.1 |
150--175 |
|
|
|
| STM0033 |
|
(AC)8 AG (AC)9 (AT)7 |
F |
AGATGTACTATCTAGCCCACGAGG |
60.1 |
184 |
3 |
|
|
| |
|
|
R |
GTCACTCATAGAGGCTATTGTGGA |
59.7 |
|
|
|
|
| STM0037 |
|
(TC)5 (AC)6 AA...(AC)7 (AT)4 |
F |
AATTTAACTTAGAAGATTAGTCTC |
47.7 |
90 |
1 |
|
XI |
| |
|
|
R |
ATTTGGTTGGGTATGATA |
48.2 |
75 |
|
|
|
| STM0038 |
|
(TA)4 (TG)12 |
F |
AACTCTAGCAGTATTTGCTTCA |
53.9 |
108 |
2 |
|
II |
| |
|
|
R |
TTATTTAGCGTCAAATGCATA |
53.9 |
|
|
|
|
| STM0040 |
|
(AT)20 AG...(AT)2 (GT)11 |
F |
GCAATAATGGCCAACACTTC |
58.1 |
140 |
3 |
|
III,XII |
| |
|
|
R |
TGGGAAATGTTAGTCAAAAATAGC |
58.7 |
85--100 |
|
|
|
| STM0045 |
|
(A)8 (TA)2 (TG)10 |
F |
TAGGAGCACACTATTGGTG |
51.9 |
159 |
3 |
|
|
| |
|
|
R |
GATATTCAAAGGATAAAACATG |
51.0 |
|
|
|
|
| STM0046 |
|
(GC)4 (AC)17 (AT)5 |
F |
TGACAAGGATAACATCAGCATAAG |
58.3 |
115 |
1 |
|
|
| |
|
|
R |
GCATTTAAGTTAGGAGTTCATGCT |
58.1 |
|
|
|
|
| STM0047 |
|
(AC)13 (AT)9 |
F |
AATTATATGCTATCACACACA |
48.3 |
165 |
2 |
|
|
| |
|
|
R |
ACTTCATTGACCACTACG |
48.6 |
|
|
|
|
| STM0050 |
|
(AC)18 |
F |
TCAGAGGTTTTGTCACGTT |
54.0 |
117 |
2 |
|
|
| |
|
|
R |
TATATGGGACACACGTGC |
53.4 |
|
|
|
|
| STM0051 |
|
(AC)7 ... (AC)7 (AT)4 |
F |
TACATACATACACACACGCG |
53.9 |
115 |
2 |
|
X |
| |
|
|
R |
CTGCAACTTATAGCCTCCA |
54.1 |
|
|
|
|
| STM0052 |
|
(AC)5 ... (AC)6....(AC)16 |
F |
TAGGCTCGGGTCATTACAATAA |
58.7 |
161 |
2 |
|
VII |
| |
|
|
R |
GCTCGCCTGTGTTTGTTGT |
59.9 |
|
|
|
|
| STM1001 |
X72927 |
(A)19 |
F |
TCAACAGTGCATTTGAATTATCG |
60.0 |
133 |
1 |
3' - UTR |
VIII |
| |
|
|
R |
TCACCTTTTCATAGTTCAGCTAGG |
59.0 |
|
|
|
|
| STM1002 |
X67511 |
(AT)11 |
F |
TATTCCCCCTTCCTACTCAA |
56.2 |
202 |
2 |
INTRON 5 |
|
| |
|
|
R |
TCTTCCACATTCCTAACCTG |
55.2 |
|
|
|
|
| STM1003 |
Z27235 |
(TA)10 |
F |
CACTTAATATGGAATAGAGGAAGTA |
53.2 |
172 |
1 |
3' - UTR |
VII |
| |
|
|
R |
GAACTTATGCTTTATCATTCA |
53.9 |
|
|
|
|
| STM1004 |
Z27235 |
(AAG)7 |
F |
ATATGAAATTCTCTCGATGTTTCG |
58.7 |
163 |
1 |
PROMOTER |
VII |
| |
|
|
R |
TCAGCCCATAAAXCTTTAGTTACCT |
57.6 |
|
|
|
|
| STM1005 |
X66826 |
(GTA)6 |
F |
ATGCCTCTTACGAATAACTCGG |
59.7 |
175 |
2 |
5' - UTR |
VIII |
| |
|
|
R |
CAGCTAACGTGGTTGGGG |
60.1 |
|
|
|
|
| STM1007 |
X57557 |
(TGG)3 |
F |
AATGTCATTCGTTACTTCCC |
54.1 |
175 |
1 |
CDS |
|
| |
|
|
R |
AGTCAAGGGAGTAAACAAAG |
51.2 |
|
|
|
|
| STM1008 |
N/A |
(AT)10 |
F |
GTACACAGCAAAATAGCAAG |
56.0 |
140 |
3 |
CP55 |
II,IV,IX |
| |
|
|
R |
TAGACACTCTCACATCCACT |
56.0 |
|
|
|
|
| STM1009 |
N/A |
(AT)30 |
F |
ATTAGCATACGACTCAAC |
45.2 |
198 |
3 |
CP54 |
VII,XI |
| |
|
|
R |
TTATTTTCATTTTTCAGC |
46.1 |
|
|
|
|
| STM1016 |
X58453 |
(TCT)9 |
F |
TTCTGATTTCATGCATGTTTCC |
59.9 |
250 |
2 |
INTRON 1 |
|
| |
|
|
R |
ATGCTTGCCATGTGATGTGT |
60.0 |
|
|
|
|
| STM1017 |
L13771 |
(ATT)5 |
F |
GACACGTTCACCATAAA |
49.7 |
132 |
2 |
5' - UTR |
IX |
| |
|
|
R |
AGAAGAATAGCAAAGCAA |
48.4 |
|
|
|
|
| STM1019 |
L01402 |
(ATC)5 |
F |
TAGATTTTATTATTCCCAACAAGCA |
58.3 |
201 |
2 |
3' - UTR |
|
| |
|
|
R |
CAACTACCTTCTCCCCACATAG |
57.8 |
|
|
|
|
| STM1020 |
M15186 |
(T)12 (A)9 ...(TA)7...(ATA)6 |
F |
TTCGTTGCTTACCTACTA |
46.5 |
216 |
3 |
INTRON 1 |
III,V |
| |
|
|
R |
CCCAAGATTACCACATTC |
50.8 |
|
|
|
|
| STM1021 |
M17108 |
(C)17..(CT)8(AT)9 |
F |
GGAGTCAAAGTTTGCTCACATC |
58.8 |
181 |
2 |
INTRON1 |
IX |
| |
|
|
R |
CACCCTCAACCCCCATATC |
60.0 |
|
|
|
|
| STM1024 |
S74753 |
(TTG)6 |
F |
ATACAGGACCTTAATTTCCCCAA |
60.0 |
143 |
1 |
5' - UTR |
VIII |
| |
|
|
R |
TCAAAACCCAATTCAATCAAATC |
60.0 |
|
|
|
|
| STM1025 |
X04118 |
(T)15 ((A)8 |
F |
ATTTCGTTGCTTACCTACTA |
49.6 |
223 |
3 |
INTRON 1 |
III |
| |
|
|
R |
AACCCAAGATTACCACATTC |
54.0 |
|
|
|
|
| STM1029 |
S51538 |
(C)12 |
F |
AGGTTCACTCACAATCAAAGCA |
59.8 |
165 |
2 |
5' - UTR |
I |
| |
|
|
R |
AAGATTTCCAAGAAATTTGAGGG |
59.8 |
|
|
|
|
| STM1030 |
X90990 |
(T)15 |
F |
GTTCATTCGGATAGACTTGAGACA |
59.7 |
177 |
2 |
INTRON |
II |
| |
|
|
R |
TGCAAATACTCTAGAGCAAGAAGG |
59.3 |
|
|
|
|
| STM1031 |
X55748 |
(AT)13 |
F |
TGTGTTTGTTTTTCTGTAT |
45.9 |
276 |
2 |
3' - UTR |
V |
| |
|
|
R |
AATTCTATCCTCATCTCTA |
43.1 |
|
|
|
|
| STM1036 |
S70185 |
(CCA)5 |
F |
GAGGACATAAGAAGACGGAG |
54.0 |
151 |
1 |
CDS |
|
| |
|
|
R |
TTTTCCTCTTCAGTCTTTGAG |
54.0 |
|
|
|
|
| STM1040 |
Z11882 |
(A)19 |
F |
AGTACTCAGTCAATCAAAG |
44.2 |
173 |
2 |
5' - UTR |
|
| |
|
|
R |
AGGTAAGTATGTTCTCCAG |
46.3 |
|
|
|
|
| STM1041 |
X73684 |
(GAA)5 |
F |
GTTGAGTAGAAGGAGGATT |
48.0 |
98 |
1 |
CDS |
V |
| |
|
|
R |
CCTTTGTCTTCTGCTTTTG |
53.6 |
|
|
|
|
| STM1043 |
U24088 |
(T)14 ... (TAA)5 |
F |
ATTTGAATTGAAGAACTTAATAGAA |
53.0 |
226 |
1 |
INTRON |
|
| |
|
|
R |
CACAAACAAAATACTGTTAACTCA |
54.1 |
|
|
|
|
| STM1045 |
X60506 |
(TGG)5 |
F |
GAAGTTTTATCAGAATCC |
46.6 |
181 |
4 |
|
II,XII |
| |
|
|
R |
ATCACCTCATCAGCAATC |
52.5 |
|
|
|
|
| STM1046 |
L22576 |
(GTG)3 ... (AGA)3 |
F |
AAAGCAGCTTGAGGATGGTC |
59.4 |
132 |
1 |
CDS |
|
| |
|
|
R |
ATCTTCTTTGGCTTGGTGTA |
55.0 |
|
|
|
|
| STM1049 |
X13497 |
(ATA)6 |
F |
CTACCAGTTTGTTGATTGTGGTG |
59.5 |
189 |
2 |
3' - UTR |
I |
| |
|
|
R |
AGGGACTTTAATTTGTTGGACG |
59.4 |
|
|
|
|
| STM1050 |
A12164 |
(AT)16 |
F |
GTACATATATACAATTATCTAACCG |
49.4 |
130 |
4 |
5 ' - UTR |
III,IV,VI, |
| |
|
|
R |
TTCTCTATGTTAGGCTAGAGTG |
51.0 |
|
|
|
VII |
| STM1051 |
N/A |
(TAT)4 TTT (TAT)7 |
F |
TCCCCTTGGCATTTTCTTCTCC |
65.7 |
225 |
2 |
|
IX |
| |
|
|
R |
TTTAGGGTGGGGTGAGGTTGG |
65.3 |
|
|
|
|
| STM1052 |
N/A |
(AT)14 GT (AT)4 (GT)6 |
F |
CAATTTCGTTTTTTCATGTGACAC |
60.6 |
248 |
2 |
|
|
| |
|
|
R |
ATGGCGTAATTTGATTTAATACGTAA |
59.2 |
|
|
|
|
| STM1053 |
U51986 |
(TA)4 (ATC)5 |
F |
TCTCCCCATCTTAATGTTTC |
54.2 |
172 |
2 |
3' - UTR |
III |
| |
|
|
R |
CAACACAGCATSCAGATCATC |
54.0 |
|
|
|
|
| STM1054 |
M18882 |
(AATT)4 |
F |
CACCAAACCTCAACAAAAG |
54.1 |
156 |
2 |
5' - UTR |
|
| |
|
|
R |
ATTTATGGTATTACTTGGGTTACA |
54.0 |
|
|
|
|
| STM1055 |
X04077 |
(AATT)5 |
F |
CACAACCAACAAGGTAAATG |
54.0 |
218 |
2 |
5' - UTR |
VIII |
| |
|
|
R |
TGTGTTAGACACCTTATTACTACG |
53.3 |
|
|
|
|
| STM1056 |
X04077 |
(AAAAT)4 |
F |
AGGTAAGTTTTATTTTCAATTGC |
53.9 |
229 |
4 |
INTRON 2 |
VIII |
| |
|
|
R |
GGGTATGGGAATAGGTAGTTT |
53.9 |
|
|
|
|
| STM1057 |
M95197 |
(AAAT)4 |
F |
TTATGTTTCGGTTAAAATGTA |
50.5 |
107 |
1 |
3' - UTR |
VIII |
| |
|
|
R |
AAATTAAATGGAAGACAACC |
51.0 |
|
|
|
|
| STM1058 |
Z13992 |
(ATT)5 |
F |
ACAATTTAATTCAAGAAGCTAGG |
54.0 |
113 |
1 |
3' - UTR |
III |
| |
|
|
R |
CCAAATTTGTATACTTCAATATGA |
53.1 |
|
|
|
|
| STM1064 |
X63093 |
(TA)12 ... (TG)4 GT (TG)5 |
F |
GTTCTTTTGGTGGTTTTCCT |
55.8 |
204 |
1 |
INTRON 1 |
II |
| (Legast) |
|
|
R |
TTATTTCTCTGTTGTTGCTG |
51.5 |
|
|
|
|
| STM1069 |
X78271 |
(TCC)4 |
F |
ATGCTAACTTGGACACTTA |
48.0 |
393 |
1 |
INTRON 8 |
III |
| (Lepdsgen) |
|
|
R |
AGTCTCTCAGGAGGATTAC |
47.7 |
|
|
|
|
| STM1072 |
X05985 |
(TG)4 (TA)6 |
F |
CATTACCTCCATTGCTA |
47.0 |
173 |
2 |
INTRON 1 |
|
| (Lerbco) |
|
|
R |
GTCTCGTACTTCTTCAT |
41.1 |
|
|
|
|
| STM1088 |
Y00478 |
(T)12(A)8 |
F |
TGGGGCTTCTTTGAG |
50.5 |
365 |
3 |
INTRON1 |
VII |
| (Leaco2) |
|
|
R |
TCCCATGGTTCACCA |
52.2 |
|
|
|
|
| Lernalx |
X79338 |
(ATT)6 |
F |
CTCACCCACAAAGAAAATTC |
54.8 |
126 |
2 |
CODING |
V |
| |
|
|
R |
CTAACAAACATTGTACAACAATAATC |
54.2 |
|
|
|
|
| LeatpacA |
M96324 |
(TA)7 |
F |
TTACTTACTCCCCTCCAACTC |
55.1 |
189 |
|
|
I |
| |
|
|
R |
CGTTTGGTTACAAGAGAATTG |
55.1 |
|
|
|
|
| STM1091 |
X67845 |
(GCT)5 |
F |
GGACAATCCATAAATCTCAG |
55.7 |
111 |
2 |
CDS |
|
| |
|
|
R |
AGCAGCAGCAGCCTCTCCTA |
66.9 |
|
|
|
|
| STM1092 |
X04753 |
(AG)11 |
F |
AGCTATGTTAGGGTCAAGGGC |
59.6 |
143 |
2 |
INTRON 1 |
|
| |
|
|
R |
CAAGGATTAAAAGCACCTC |
52.5 |
|
|
|
|
| STM1093 |
Z12824 |
(TA)23 |
F |
TGTACTGGGGAGCCTCAAAG |
60.0 |
148 |
2 |
3' - UTR |
|
| |
|
|
R |
AATTTTAACCTCGTGACATGGG |
60.0 |
|
|
|
|
| STM1097 |
U24088 |
(CGTTT)5 |
F |
TGATTTAGTTGCTTGTTTG |
54.1 |
124 |
1 |
INTRON 1 |
|
| |
|
|
R |
GCTTTCGATCCTAATACACC |
54.0 |
|
|
|
|
| STM1100 |
U23757 |
(TA)22 |
F |
GCTTCGCCATCTTAATGGTTA |
59.2 |
173 |
2 |
3' - UTR |
VI |
| |
|
|
R |
TCTTCGAAGGTACGTTTAGACAA |
58.1 |
|
|
|
|
| STM1102 |
J03679 |
(TA)8 |
F |
GGAAGAATTTTGTAGGTTCAA |
53.7 |
167 |
2 |
5' - UTR |
I, IX |
| |
|
|
R |
AAAGTGAAACTTCCTAGCATG |
53.9 |
|
|
|
|
| STM1104 |
X52417 |
(TCT)5 |
F |
TGATTCTCTTGCCTACTGTAATCG |
59.8 |
168 |
1 |
3' - UTR |
VIII |
| |
|
|
R |
CAAAGTGGTGTGAAGCTGTGA |
59.9 |
|
|
|
|
| STM1105 |
X52416 |
(ACTC)6 |
F |
AAACCTGCTACAAATAAGGC |
53.9 |
114 |
2 |
5' - UTR |
VIII |
| |
|
|
R |
CAGAAATAATTGGAGGAGATG |
54.0 |
|
|
|
|
| STM1106 |
N/A |
(ATT)13 |
F |
TCCAGCTGATTGGTTAGGTTG |
60.1 |
156 |
2 |
INTRON |
X |
| |
|
|
R |
ATGCGAATCTACTCGTCATGG |
60.1 |
|
|
|
|
| STM2002 |
|
(TTG)3 .. (AC)3 |
F |
AGAACCATTTGATGCATATCCC |
60.0 |
159 |
1 |
|
|
| |
|
|
R |
TTGTAAGTTTGGGATGAAGCG |
60.1 |
|
|
|
|
| STM2003 |
|
(AAT)3..(AT)3..(TC)3..(TA)3 |
F |
TTTTAATCCAACCAATCTTTAGCC |
59.8 |
151 |
1 |
|
|
| |
|
|
R |
AGCATTTTGCATCCTGTGTTT |
59.6 |
|
|
|
|
| STM2004 |
|
(CAA)6 |
F |
ACCAGCGCAATAACTCCG |
59.8 |
130 |
1 |
|
|
| |
|
|
R |
GCTTTGTGGATTCACCGG |
60.0 |
|
|
|
|
| STM2005 |
|
(CTGTTG)3 |
F |
TTTAAGTTCTCAGTTCTGCAGGG |
59.9 |
166 |
1 |
|
XI |
| |
|
|
R |
GTCATAACCTTTACCATTGCTGG |
59.8 |
|
|
|
|
| STM2007 |
|
(TTG)3..(TC)3..(CTT)3 |
F |
GAAATGGCACAAATCAGCATT |
58.0 |
179 |
3 |
|
|
| |
|
|
R |
GCAGAAACAAGTAGAGAAGAAGCC |
58.0 |
|
|
|
|
| STM2010 |
|
(CT)5..(AG)3 |
F |
GAATTCGCGGCCGCGATCAC |
60.0 |
253 |
2 |
|
|
| |
|
|
R |
TCAAGGTCACCGACACGATAC |
61.0 |
|
|
|
|
| STM2012 |
|
(CA)3..(GA)4..(AG)3..(CGG)5 |
F |
GCGGCCGCTTCTCAGCCAA |
64.0 |
247 |
2 |
|
X |
| |
|
|
R |
TCTCGTTCAATCCACCAGATC |
60.0 |
|
|
|
|
| STM2013 |
|
(TCTA)6 |
F |
TTCGGAATTACCCTCTGCC |
60.0 |
160 |
2 |
|
VII |
| |
|
|
R |
AAAAAAAGAACGCGCACG |
60.0 |
|
|
|
|
| STM2014 |
|
(CAA)4..(CAA)5 |
F |
TTCATGAGACCACTTTCAAGTTTC |
59.7 |
202 |
1 |
|
|
| |
|
|
R |
GTTCTCATGGAAGAGGCAACA |
60.2 |
|
|
|
|
| STM2017 |
|
(CAG)3..(CAG)4 |
F |
TGGCGATGCAGCAGTATC |
59.6 |
160 |
2 |
|
|
| |
|
|
R |
TTCTGAATCTGAGTCGGCG |
60.1 |
|
|
|
|
| STM2018 |
|
(TA)7 |
F |
CCAAAGAGGTATCCCACTACTCCT |
59.1 |
170 |
2 |
|
|
| |
|
|
R |
TTTGGACACAAAGGGAAGTTG |
60.0 |
|
|
|
|
| STM2020 |
|
(TAA)6 |
F |
CCTTCCCCTTAAATACAATAACCC |
60.2 |
162 |
1 |
|
I |
| |
|
|
R |
CATGGAGAAGTGAAAACGTCTG |
59.8 |
|
|
|
|
| STM2021 |
|
(GA)6 |
F |
AACGTTTGACTAAGCCCCAGT |
60.0 |
169 |
1 |
|
|
| |
|
|
R |
TTGGTATGGGGGATAAAGTTCA |
60.0 |
|
|
|
|
| STM2022 |
|
(CAA)3..(CAA)3 |
F |
GCGTCAGCGATTTCAGTACTA |
58.2 |
194 |
2 |
|
II |
| |
|
|
R |
TTCAGTCAACTCCTGTTGCG |
60.0 |
|
|
|
|
| STM2023 |
|
(TCC)3..(TC)3..(TC)3 |
F |
TTTTCCGACGAAATCCTCC |
60.0 |
162 |
1 |
|
|
| |
|
|
R |
GAAAAAAGGAGTAGAGGCGGC |
60.3 |
|
|
|
|
| STM2024 |
|
(GA)4..(CAA)4..(CAG)3 |
F |
GGCCGCAAAAAAACCATAC |
60.3 |
160 |
1 |
|
|
| |
|
|
R |
GCCATCCATTGCTGCTGA |
62.0 |
|
|
|
|
| STM2026 |
|
(CT)4..(CT)5 |
F |
AATCCCTTTTGTAGTCTTTTGCTT |
58.9 |
191 |
1 |
|
|
| |
|
|
R |
TGGTGACACTCCATTCAAACA |
60.0 |
|
|
|
|
| STM2028 |
|
(TAC)5...(TA)3....(CAT)3 |
F |
TCTCACCAGCCGGAACAT |
60.2 |
188 |
2 |
|
XII |
| |
|
|
R |
AAGCTGCGGAAGTGATTTTG |
60.4 |
|
|
|
|
| STM2030 |
|
(CA)3(TA)5 |
F |
TCTTCCCAAATCTAGAATACATGC |
58.7 |
176 |
2 |
|
I |
| |
|
|
R |
AAAGTTAGCATGGACAGCATTTC |
59.7 |
|
|
|
|
| STM3000 |
|
(TC)13 |
F |
CAACCCAATTTCGAGAGGGAC |
68.0 |
116 |
2 |
|
|
| |
|
|
R |
TGTCCCAAAATCGTCTCTGGC |
66.0 |
|
|
|
|
| STM3001 |
|
(TC)5 |
F |
ATCGGAGCACCCATCGGAAG |
64.0 |
133 |
3 |
|
II |
| |
|
|
R |
GATCGCCCAAATCGGTTGAG |
62.0 |
|
|
|
|
| STM3003 |
|
(CT)4, (CA) |
F |
CATAAATCACTAAAACTAGATAACC |
64.0 |
317 |
1 |
|
|
| |
|
|
R |
TATTTCATATCAATGTCGAATCCC |
62.0 |
|
|
|
|
| STM3009 |
|
(TC)13 |
F |
TCAGCTGAACGACCACTGTTC |
64.0 |
110 |
2 |
|
VII |
| |
|
|
R |
GATTTCACCAAGCATGGAAGTC |
64.0 |
140 |
|
|
|
| STM3010 |
|
(CT)14 |
F |
TCATGAGTGCTGGTGCTACC |
59.9 |
120 |
2 |
|
VIII |
| |
|
|
R |
GTCTTAACAGCAAATCAACAATGG |
59.9 |
|
|
|
|
| STM3011 |
|
(CT)10..(CT)16..(AC)5 |
F |
GTGTGGTTGATTGATTGATTTAGC |
59.8 |
125 |
3 |
|
II |
| |
|
|
R |
GTTTTTAGGCAGTTCTTGGGG |
60.0 |
|
|
|
|
| STM3012 |
|
(CT)4..(CT)8 |
F |
CAACTCAAACCAGAAGGCAAA |
60.3 |
193 |
1 |
|
IX |
| |
|
|
R |
GAGAAATGGGCACAAAAAACA |
60.0 |
|
|
|
|
| STM3015 |
|
(CT)11..(CA)12 |
F |
AGCAATAAAGTCAACACTCCATCA |
66.0 |
156 |
2 |
|
VIII |
| |
|
|
R |
AATGAATTAGGGGGAGGTGTG |
62.0 |
|
|
|
|
| STM3016 |
|
(GA)27 |
F |
TCAGAACACCGAATGGAAAAC |
60.0 |
151 |
2 |
|
|
| |
|
|
R |
GCTCCAACTTACTGGTCAAATCC |
60.0 |
|
|
|
|
| STM3018 |
|
(GA)9 |
F |
CCAAAAGTCCGGCAAACTT |
60.1 |
168 |
1 |
|
II |
| |
|
|
R |
CCATATGAGGCCCCTTTTTAC |
59.7 |
|
|
|
|
| STM3020 |
|
(CT)10 |
F |
TTTTACGGTGAAGCCACTCC |
60.1 |
178 |
2 |
|
|
| |
|
|
R |
CTTGGGGTTGCCAGTTTG |
60.1 |
|
|
|
|
| STM3023 |
|
(GA)9..(GA)8..(GA)4 |
F |
AAGCTGTTACTTGATTGCTGCA |
60.1 |
197 |
2 |
|
|
| |
|
|
R |
GTTCTGGCATTTCCATCTAGAGA |
59.1 |
|
|
|
|
